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pCDF-His_TEV_MsbA
(Plasmid #191476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF-Duet-1
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3429
  • Total vector size (bp) 5232
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MsbA
  • Species
    E. coli
  • Insert Size (bp)
    1803
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF-His_TEV_MsbA was a gift from Arthur Laganowsky (Addgene plasmid # 191476 ; http://n2t.net/addgene:191476 ; RRID:Addgene_191476)

  • For your References section:

    Structural basis for lipid and copper regulation of the ABC transporter MsbA. Lyu J, Liu C, Zhang T, Schrecke S, Elam NP, Packianathan C, Hochberg GKA, Russell D, Zhao M, Laganowsky A. Nat Commun. 2022 Nov 26;13(1):7291. doi: 10.1038/s41467-022-34905-2. 10.1038/s41467-022-34905-2 PubMed 36435815
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