pACE-K2P4.1-StrepTagII
(Plasmid
#191474)
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PurposeExpresses K2P4.1 with C-terminal StrepTagII
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191474 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACEBAC1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2682
- Total vector size (bp) 3555
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameK2P4.1
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Alt nameTRAAK
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SpeciesH. sapiens (human)
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Insert Size (bp)918
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MutationN104Q N108Q
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Entrez GeneKCNK4 (a.k.a. FHEIG, K2p4.1, TRAAK, TRAAK1)
- Promoter polH
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer polH
- 3′ sequencing primer SV40_R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pACE-K2P4.1-StrepTagII was a gift from Arthur Laganowsky (Addgene plasmid # 191474 ; http://n2t.net/addgene:191474 ; RRID:Addgene_191474) -
For your References section:
Cupric Ions Selectively Modulate TRAAK-Phosphatidylserine Interactions. Zhu Y, Schrecke S, Tang S, Odenkirk MT, Walker T, Stover L, Lyu J, Zhang T, Russell D, Baker ES, Yan X, Laganowsky A. J Am Chem Soc. 2022 Apr 27;144(16):7048-7053. doi: 10.1021/jacs.2c00612. Epub 2022 Apr 14. 10.1021/jacs.2c00612 PubMed 35421309