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Addgene

pACE-K2P4.1-StrepTagII
(Plasmid #191474)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191474 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pACEBAC1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2682
  • Total vector size (bp) 3555
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    K2P4.1
  • Alt name
    TRAAK
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    918
  • Mutation
    N104Q N108Q
  • Entrez Gene
    KCNK4 (a.k.a. FHEIG, K2p4.1, TRAAK, TRAAK1)
  • Promoter polH

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACE-K2P4.1-StrepTagII was a gift from Arthur Laganowsky (Addgene plasmid # 191474 ; http://n2t.net/addgene:191474 ; RRID:Addgene_191474)
  • For your References section:

    Cupric Ions Selectively Modulate TRAAK-Phosphatidylserine Interactions. Zhu Y, Schrecke S, Tang S, Odenkirk MT, Walker T, Stover L, Lyu J, Zhang T, Russell D, Baker ES, Yan X, Laganowsky A. J Am Chem Soc. 2022 Apr 27;144(16):7048-7053. doi: 10.1021/jacs.2c00612. Epub 2022 Apr 14. 10.1021/jacs.2c00612 PubMed 35421309