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Addgene

pFR-mTq2-antiFRET-P2A-R-GECO1
(Plasmid #191471)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191471 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFR
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    R-GECO1
  • Species
    Synthetic
  • Tag / Fusion Protein
    • His-mTurquoise2-antiFRETlinker-P2A (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFR-mTq2-antiFRET-P2A-R-GECO1 was a gift from Dorus Gadella (Addgene plasmid # 191471 ; http://n2t.net/addgene:191471 ; RRID:Addgene_191471)

  • For your References section:

    A green lifetime biosensor for calcium that remains bright over its full dynamic range. van der Linden FH, Thornquist SC, ter Beek RM, Huijts JY, Hink MA, Gadella Jr. TWJ, Maimon G, Goedhart J.. bioRxiv 2024.11.05.622032; doi: https://doi.org/10.1101/2024.11.05.622032 10.1101/2024.11.05.622032
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