4xmts-G-Ca-FLITS
(Plasmid
#191462)
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PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191462 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG-Ca-FLITS
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SpeciesSynthetic
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Tag
/ Fusion Protein
- 4xmts (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site BsrGI (not destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
4xmts-G-Ca-FLITS was a gift from Dorus Gadella (Addgene plasmid # 191462 ; http://n2t.net/addgene:191462 ; RRID:Addgene_191462) -
For your References section:
A green lifetime biosensor for calcium that remains bright over its full dynamic range. van der Linden FH, Thornquist SC, ter Beek RM, Huijts JY, Hink MA, Gadella Jr. TWJ, Maimon G, Goedhart J.. bioRxiv 2024.11.05.622032; doi: https://doi.org/10.1101/2024.11.05.622032 10.1101/2024.11.05.622032
