pMYC BRGI aa597-1407
(Plasmid #19146)

• Depositing Lab: Joan Massague
• Publication: Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4294
• Total vector size (bp): 6738
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
• Alt name: SMARCA4, BRG1; SNF2; SWI2; BAF190; SNF2L4; SNF2LB; hSNF2b; FLJ39786; SNF2-BETA
• Species: H. sapiens (human)
• Insert Size (bp): 2444
• Mutation: human BRG1 aa 597-1407 (PCR product)
• Entrez Gene: SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: NcoI (not destroyed)
• 5′ sequencing primer: M13 pUC reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Qiaoran Xi

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Myc tagged human BRG1 cDNA truncated form from aa 597 to 1407