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Addgene

nanobody(AntiGFP)-HaloTag-iLID
(Plasmid #191453)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191453 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nanobody(AntiGFP)-HaloTag-iLID
  • Species
    Synthetic

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    nanobody(AntiGFP)-HaloTag-iLID was a gift from Dorus Gadella (Addgene plasmid # 191453 ; http://n2t.net/addgene:191453 ; RRID:Addgene_191453)

  • For your References section:

    iLID-antiGFP-nanobody is a flexible targeting strategy for recruitment to GFP-tagged proteins. Mahlandt EK, Toereppel M, Haydary T, Goedhart J. bioRxiv 2022.11.24.517828 10.1101/2022.11.24.517828
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