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pMYC BRGI aa1400-1648
(Plasmid #19145)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19145 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
  • Alt name
    SMARCA4, BRG1; SNF2; SWI2; BAF190; SNF2L4; SNF2LB; hSNF2b; FLJ39786; SNF2-BETA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    700
  • Mutation
    human BRG1 aa 1400-1648 (PCR product)
  • Entrez Gene
    SMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer M13 pUC reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Qiaoran Xi

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Myc tagged human BRG1 cDNA truncated form from aa 1400 to 1648

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMYC BRGI aa1400-1648 was a gift from Joan Massague (Addgene plasmid # 19145 ; http://n2t.net/addgene:19145 ; RRID:Addgene_19145)

  • For your References section:

    Genome-wide impact of the BRG1 SWI/SNF chromatin remodeler on the transforming growth factor beta transcriptional program. Xi Q, He W, Zhang XH, Le HV, Massague J. J Biol Chem. 2008 Jan 11. 283(2):1146-55. 10.1074/jbc.M707479200 PubMed 18003620
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