Epac-SH225
(Plasmid
#191449)
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Purposeexpression of cAMP sensor in yeast
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemT2Del-EPACdDEPCD-tdTomato
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SpeciesH. sapiens (human)
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Insert Size (bp)4335
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
tdTomato might have maturation problems in certain type of cells. Only measure cells with fully maturated tdTomato.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Epac-SH225 was a gift from Kees Jalink (Addgene plasmid # 191449 ; http://n2t.net/addgene:191449 ; RRID:Addgene_191449)