p5E-pth2
(Plasmid #191428)

• Purpose: Tol2 Gateway 5' entry vector containing 2067 bp upstream of the CDS for zebrafish pth2 (ENSDARG00000022951), including the 5' UTR and first intron (GRCz11 chr 17:33493999-33496066)
• Depositing Lab: Timothy Erickson
• Publication: Venuto et al Biol Open. 2022 Sep 26. pii: 276788. doi: 10.1242/bio.059432.

Backbone

• Vector backbone: pDONR-P4-P1R
• Backbone size w/o insert (bp): 4777
• Total vector size (bp): 4712
• Vector type: Tol2 Gateway 5' entry vector

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pth2 regulatory region
• Species: D. rerio (zebrafish)
• Insert Size (bp): 2067
• GenBank ID: NM_205577
• Entrez Gene: pth2 (a.k.a. tip39)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Supplemental Documents:
  • p5e-pth2.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • National Institute of Genetics Limited Use Label License for Tol2 System
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.05.06.490781v3 for bioRxiv preprint.