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hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)
(Plasmid #191391)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2364
  • Total vector size (bp) 7193
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hCdt1(30-120)
  • Insert Size (bp)
    999
  • Tag / Fusion Protein
    • mScarlet-I (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

-Expression is driven by the human PGK1 promoter and BGH polyA

-Use in combination with eSpCas9(1.1)_NoFlag_hROSA26_Left (Plasmid #191442)

-Cdt1(30-120) sequence is derived from the original FUCCI system PMID: 18267078

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120) was a gift from Yannick Doyon (Addgene plasmid # 191391 ; http://n2t.net/addgene:191391 ; RRID:Addgene_191391)
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