hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)
(Plasmid
#191389)
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PurposeDonor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191389 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
- Backbone size w/o insert (bp) 2364
- Total vector size (bp) 7220
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehCdt1(1-100)
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SpeciesH. sapiens (human)
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Insert Size (bp)999
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Tag
/ Fusion Protein
- mScarlet-I (N terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
--Expression is driven by the human PGK1 promoter and BGH polyA
-Use in combination with eSpCas9(1.1)_NoFlag_hROSA26_Left (Plasmid #191442)
-hCdt1(1-100) sequence is derived from the Fucci(CA) system PMID: 29107535
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100) was a gift from Yannick Doyon (Addgene plasmid # 191389 ; http://n2t.net/addgene:191389 ; RRID:Addgene_191389)
