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Addgene

pSLIK-rABE-Flag
(Plasmid #191383)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191383 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSLIK
  • Backbone size w/o insert (bp) 12648
  • Total vector size (bp) 13200
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    rABE-Flag
  • Alt name
    RNA Adenosine Base Editor
  • Species
    Synthetic
  • Insert Size (bp)
    552
  • Promoter tet-inducible promoter
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer TCAGATCGCCACTAGTCCACCATGTCTGAGGTGGAATTC
  • 3′ sequencing primer GCTGGGTCTAGATATCTCGAGTCACTTGTCATCGTCATCCTTGTAATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLIK-rABE-Flag was a gift from Stephen Floor (Addgene plasmid # 191383 ; http://n2t.net/addgene:191383 ; RRID:Addgene_191383)

  • For your References section:

    RNA molecular recording with an engineered RNA deaminase. Lin Y, Kwok S, Hein AE, Thai BQ, Alabi Y, Ostrowski MS, Wu K, Floor SN. Nat Methods. 2023 Dec;20(12):1887-1899. doi: 10.1038/s41592-023-02046-z. Epub 2023 Oct 19. 10.1038/s41592-023-02046-z PubMed 37857907
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