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pMDJ231 - Phsp Cas9
(Plasmid #191382)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191382 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDESTR4-R3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2381
  • Total vector size (bp) 10509
  • Vector type
    Worm Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Phsp-16.41 Cas9 gpd-2 tagRFP-t
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    8121
  • Promoter Phsp-16.41

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDJ231 - Phsp Cas9 was a gift from Christian Froekjaer-Jensen (Addgene plasmid # 191382 ; http://n2t.net/addgene:191382 ; RRID:Addgene_191382)

  • For your References section:

    Modular safe-harbor transgene insertion (MosTI) for targeted single-copy and extrachromosomal array integration in C. elegans. El Mouridi S, Alkhaldi F, Frokjaer-Jensen C. G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 10.1093/g3journal/jkac184. 10.1093/g3journal/jkac184 PubMed 35900171
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