pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS
(Plasmid
#191369)
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PurposeOverexpression of NLS-NLS-dPba3Cas13b-3xGFP-NLS in human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191369 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHAGE
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNLS-NLS-dPba3Cas13b-3xGFP-NLS
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SpeciesSynthetic
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Tag
/ Fusion Protein
- 3xGFP (C terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHAGE-IRES-puro-NLS-NLS-dPba3Cas13b-3xGFP-NLS was a gift from Ling-Ling Chen (Addgene plasmid # 191369 ; http://n2t.net/addgene:191369 ; RRID:Addgene_191369) -
For your References section:
Multi-color RNA imaging with CRISPR-Cas13b systems in living cells. Yang LZ, Gao BQ, Huang Y, Wang Y, Yang L, Chen LL. Cell Insight. 2022 Jun 15;1(4):100044. doi: 10.1016/j.cellin.2022.100044. eCollection 2022 Aug. 10.1016/j.cellin.2022.100044 PubMed 37192858