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Addgene

CD23b in pIRES2eGFP
(Plasmid #19136)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19136 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pIRES2eGFP
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5300
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CD23 beta
  • Alt name
    CD23b
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    993
  • GenBank ID
    NM_002002
  • Entrez Gene
    FCER2 (a.k.a. BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF)
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD23b in pIRES2eGFP was a gift from Zena Werb (Addgene plasmid # 19136 ; http://n2t.net/addgene:19136 ; RRID:Addgene_19136)

  • For your References section:

    The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10. Lemieux GA, Blumenkron F, Yeung N, Zhou P, Williams J, Grammer AC, Petrovich R, Lipsky PE, Moss ML, Werb Z. J Biol Chem. 2007 May 18. 282(20):14836-44. 10.1074/jbc.M608414200 PubMed 17389606
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