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pQnl_Pcphy23
(Plasmid #191354)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191354 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQexp
  • Backbone manufacturer
    PMID 19775243
  • Backbone size w/o insert (bp) 6972
  • Total vector size (bp) 5776
  • Modifications to backbone
    Strong, constitutive Clostridium phytofermentans promoter (Pcons17) driving expression of NanoLuc reporter
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcphy23-NanoLuc
  • Species
    Synthetic
  • Insert Size (bp)
    589
  • Promoter Pcphy23

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI (destroyed during cloning)
  • 3′ cloning site BsmBI (destroyed during cloning)
  • 5′ sequencing primer TTACCGCCTTTGAGTGAGCT
  • 3′ sequencing primer TTGCTTCTAAGGTCGACGGA
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Promega

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQnl_Pcphy23 was a gift from Andrew Tolonen (Addgene plasmid # 191354 ; http://n2t.net/addgene:191354 ; RRID:Addgene_191354)

  • For your References section:

    Tuning of Gene Expression in Clostridium phytofermentans Using Synthetic Promoters and CRISPRi. Rostain W, Zaplana T, Boutard M, Baum C, Tabuteau S, Sanitha M, Ramya M, Guss A, Ettwiller L, Tolonen AC. ACS Synth Biol. 2022 Dec 16;11(12):4077-4088. doi: 10.1021/acssynbio.2c00385. Epub 2022 Nov 25. 10.1021/acssynbio.2c00385 PubMed 36427328
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