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Addgene

pBetaActin-FKBP-Halo-Dync1h1motor
(Plasmid #191333)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191333 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    P_actin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FKBP-Halo-Dync1h1motor
  • Species
    M. musculus (mouse)
  • Mutation
    DYNC1H1 motor domain aa 1453-4644
  • Entrez Gene
    Dync1h1 (a.k.a. 9930018I23Rik, DHC1, DHC1a, DNCL, Dnchc1, Dnec1, Dnecl, Loa, MAP1C, P22, Swl, mKIAA0325)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBetaActin-FKBP-Halo-Dync1h1motor was a gift from Frank Bradke (Addgene plasmid # 191333 ; http://n2t.net/addgene:191333 ; RRID:Addgene_191333)

  • For your References section:

    Microtubule retrograde flow retains neuronal polarization in a fluctuating state. Schelski M, Bradke F. Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. Epub 2022 Nov 4. 10.1126/sciadv.abo2336 PubMed 36332023
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