pBetaActin-mNeonGreen-CAMSAP3
(Plasmid
#191322)
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PurposeExpresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneP_actin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen-CAMSAP3
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SpeciesM. musculus (mouse)
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Entrez GeneCamsap3 (a.k.a. 2310057J16Rik, Kiaa1543, Nezha)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBetaActin-mNeonGreen-CAMSAP3 was a gift from Frank Bradke (Addgene plasmid # 191322 ; http://n2t.net/addgene:191322 ; RRID:Addgene_191322) -
For your References section:
Microtubule retrograde flow retains neuronal polarization in a fluctuating state. Schelski M, Bradke F. Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. Epub 2022 Nov 4. 10.1126/sciadv.abo2336 PubMed 36332023
