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Addgene

pBetaActin-TUBB2a-mEos3.2
(Plasmid #191320)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191320 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    P_actin
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TUBB2a-mEos3.2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tubb2a (a.k.a. M(beta)2, Tubb2)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBetaActin-TUBB2a-mEos3.2 was a gift from Frank Bradke (Addgene plasmid # 191320 ; http://n2t.net/addgene:191320 ; RRID:Addgene_191320)
  • For your References section:

    Microtubule retrograde flow retains neuronal polarization in a fluctuating state. Schelski M, Bradke F. Sci Adv. 2022 Nov 4;8(44):eabo2336. doi: 10.1126/sciadv.abo2336. Epub 2022 Nov 4. 10.1126/sciadv.abo2336 PubMed 36332023