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PiggyBac dCas9-KRAB
(Plasmid #191314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    piggyBac
  • Total vector size (bp) 14311
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    KRAB-dCas9-HA-tagBFP
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    5234
  • Tags / Fusion Proteins
    • HA (C terminal on insert)
    • tagBFP (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    rtTA3-IRES-PURO
  • Species
    Synthetic
  • Insert Size (bp)
    1939

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid was cloned by Dr. Daniel Fuentes.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PiggyBac dCas9-KRAB was a gift from Joanna Wysocka (Addgene plasmid # 191314 ; http://n2t.net/addgene:191314 ; RRID:Addgene_191314)

  • For your References section:

    Systematic perturbation of retroviral LTRs reveals widespread long-range effects on human gene regulation. Fuentes DR, Swigut T, Wysocka J. Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989. 10.7554/eLife.35989 PubMed 30070637
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