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Addgene

pGL3-FLIP pr5
(Plasmid #19130)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19130 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL3-basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIP promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    784
  • Mutation
    The sequence corresponds to positions -503 to +281 relative to the proposed transcriptional start site of CFLAR (FLIP) exon 1
  • Entrez Gene
    CFLAR (a.k.a. CASH, CASP8AP1, CLARP, Casper, FLAME, FLAME-1, FLAME1, FLIP, I-FLICE, MRIT, c-FLIP, c-FLIPL, c-FLIPR, c-FLIPS, cFLIP)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL3-FLIP pr5 was a gift from Wafik El-Deiry (Addgene plasmid # 19130 ; http://n2t.net/addgene:19130 ; RRID:Addgene_19130)
  • For your References section:

    Direct repression of FLIP expression by c-myc is a major determinant of TRAIL sensitivity. Ricci MS, Jin Z, Dews M, Yu D, Thomas-Tikhonenko A, Dicker DT, El-Deiry WS. Mol Cell Biol. 2004 Oct . 24(19):8541-55. 10.1128/MCB.24.19.8541-8555.2004 PubMed 15367674