pBabepuro-myc-ER
(Plasmid #19128)

• Depositing Lab: Wafik El-Deiry
• Publication: Ricci et al Mol Cell Biol. 2004 Oct . 24(19):8541-55.

Backbone

• Vector backbone: pBabepuro3:hbER tam
• Backbone size w/o insert (bp): 5169
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: c-myc
• Species: H. sapiens (human)
• Entrez Gene: MYC (a.k.a. MRTL, MYCC, bHLHe39, c-Myc)
• Tag / Fusion Protein:
  • mouse ER hormone binding domain (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pBABE-5
• 3′ sequencing primer: pBABE-3

Resource Information

• Articles Citing this Plasmid:
  • 15 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see Littlewood et al., Nucleic Acids Res. 1995 May 25;23(10):1686-90 for more information regarding the mutant hormone binding domain of the mouse estrogen receptor (hbER Tam).