PiggyBac dCas9-VPR
(Plasmid #191269)

• Purpose: piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP
• Depositing Lab: Joanna Wysocka
• Publication: Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10.7554/eLife.35989.

Backbone

• Vector backbone: piggyBac
• Total vector size (bp): 15639
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: VPR-dCas9-HA-tagBFP
• Species: Synthetic; Streptococcus pyogenes
• Tags / Fusion Proteins:
  • HA (C terminal on insert)
  • tagBFP (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAGT

Gene/Insert 2

• Gene/Insert name: rtTA3-IRES-PURO
• Species: Synthetic
• Insert Size (bp): 1939

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: AAATTTGTGAAAGATTGAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was cloned by Dr. Daniel Fuentes.