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Addgene

P74-26 Tail Tube Protein (gp93) in pSMT3
(Plasmid #191261)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191261 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSMT3
  • Backbone size w/o insert (bp) 5599
  • Total vector size (bp) 6646
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    We recommend expressing the plasmid in ArcticExpress (DE3) cells.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    P74-26 gp93
  • Alt name
    P74-26 Tail Tube Protein
  • Alt name
    P74-26 TTP
  • Species
    Thermus Virus P74-26
  • Insert Size (bp)
    1044
  • GenBank ID
    EU100884.1
  • Promoter T7
  • Tag / Fusion Protein
    • His-SUMO (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GATCGGATCCATGCGTGGTGTGGACACC
  • 3′ sequencing primer GATCCTCGAGTTAGAATTTCGCAACGTGGGTCAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P74-26 Tail Tube Protein (gp93) in pSMT3 was a gift from Brian Kelch (Addgene plasmid # 191261 ; http://n2t.net/addgene:191261 ; RRID:Addgene_191261)

  • For your References section:

    Conformational dynamics control assembly of an extremely long bacteriophage tail tube. Agnello E, Pajak J, Liu X, Kelch BA. J Biol Chem. 2023 Feb 13:103021. doi: 10.1016/j.jbc.2023.103021. 10.1016/j.jbc.2023.103021 PubMed 36791911
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