pBAD-LSSmApple
(Plasmid
#191259)
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PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein LSSmApple
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191259 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBAD
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLSSmApple
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SpeciesSynthetic
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Insert Size (bp)700
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Tag
/ Fusion Protein
- His tag (N terminal on backbone)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD-LSSmApple was a gift from Robert Campbell (Addgene plasmid # 191259 ; http://n2t.net/addgene:191259 ; RRID:Addgene_191259) -
For your References section:
A Monochromatically Excitable Green-Red Dual-Fluorophore Fusion Incorporating a New Large Stokes Shift Fluorescent Protein. Ejike JO, Sadoine M, Shen Y, Ishikawa Y, Sunal E, Hansch S, Hamacher AB, Frommer WB, Wudick MM, Campbell RE, Kleist TJ. Biochemistry. 2024 Jan 2;63(1):171-180. doi: 10.1021/acs.biochem.3c00451. Epub 2023 Dec 19. 10.1021/acs.biochem.3c00451 PubMed 38113455
