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pET28-MBP-CI2F
(Plasmid #191254)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191254 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone size w/o insert (bp) 5078
  • Total vector size (bp) 6473
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Chymotrypsin Inhibitor 2
  • Alt name
    CI2
  • Species
    Hordeum vulgare
  • Insert Size (bp)
    1395
  • Tag / Fusion Protein
    • Maltose Binding Protein (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-MBP-CI2F was a gift from Arthur Laganowsky (Addgene plasmid # 191254 ; http://n2t.net/addgene:191254 ; RRID:Addgene_191254)

  • For your References section:

    Evidence for Many Unique Solution Structures for Chymotrypsin Inhibitor 2: A Thermodynamic Perspective Derived from vT-ESI-IMS-MS Measurements. Raab SA, El-Baba TJ, Woodall DW, Liu W, Liu Y, Baird Z, Hales DA, Laganowsky A, Russell DH, Clemmer DE. J Am Chem Soc. 2020 Oct 14;142(41):17372-17383. doi: 10.1021/jacs.0c05365. Epub 2020 Sep 29. 10.1021/jacs.0c05365 PubMed 32866376
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