pET28-MBP-CI2F
(Plasmid
#191254)
-
PurposeExpresses chymotrypsin inhibitor with an N-terminal MBP fusion protein
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191254 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepET28
- Backbone size w/o insert (bp) 5078
- Total vector size (bp) 6473
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameChymotrypsin Inhibitor 2
-
Alt nameCI2
-
SpeciesHordeum vulgare
-
Insert Size (bp)1395
-
Tag
/ Fusion Protein
- Maltose Binding Protein (N terminal on insert)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET28-MBP-CI2F was a gift from Arthur Laganowsky (Addgene plasmid # 191254 ; http://n2t.net/addgene:191254 ; RRID:Addgene_191254) -
For your References section:
Evidence for Many Unique Solution Structures for Chymotrypsin Inhibitor 2: A Thermodynamic Perspective Derived from vT-ESI-IMS-MS Measurements. Raab SA, El-Baba TJ, Woodall DW, Liu W, Liu Y, Baird Z, Hales DA, Laganowsky A, Russell DH, Clemmer DE. J Am Chem Soc. 2020 Oct 14;142(41):17372-17383. doi: 10.1021/jacs.0c05365. Epub 2020 Sep 29. 10.1021/jacs.0c05365 PubMed 32866376
