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Addgene

PALI1_pHIVEGFP
(Plasmid #191252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191252 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHIV-EGFP
  • Backbone manufacturer
    addgene Plasmid #21373
  • Backbone size w/o insert (bp) 6900
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LCoR
  • Alt name
    PALI1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4671
  • Mutation
    NA
  • GenBank ID
    NG_051561 84458
  • Promoter EF1alpha
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer 5'-TGGAATTTGCCCTTTTTGAG-3'
  • 3′ sequencing primer 5'-AGGAACTGCTTCCTTCACGA-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PALI1_pHIVEGFP was a gift from Chen Davidovich (Addgene plasmid # 191252 ; http://n2t.net/addgene:191252 ; RRID:Addgene_191252)

  • For your References section:

    PALI1 facilitates DNA and nucleosome binding by PRC2 and triggers an allosteric activation of catalysis. Zhang Q, Agius SC, Flanigan SF, Uckelmann M, Levina V, Owen BM, Davidovich C. Nat Commun. 2021 Jul 28;12(1):4592. doi: 10.1038/s41467-021-24866-3. 10.1038/s41467-021-24866-3 PubMed 34321472
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