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Addgene

shLuc pRRL
(Plasmid #19125)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19125 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL-U6-siluc-cppt-hPGKGFPSIN
  • Backbone size (bp) 7247
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C, grows slowly.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    control shRNA
  • Insert Size (bp)
    60

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer EGFP N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Non specific shRNA for control experiments. Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence: 5'-CCGGTCCTAAGGTTAAGTCGCCCTCGCTCGAGCGAGGGCGACTTAACCTTAGGTTTTTG-3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    shLuc pRRL was a gift from Bob Weinberg (Addgene plasmid # 19125 ; http://n2t.net/addgene:19125 ; RRID:Addgene_19125)

  • For your References section:

    Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression. Godar S, Ince TA, Bell GW, Feldser D, Donaher JL, Bergh J, Liu A, Miu K, Watnick RS, Reinhardt F, McAllister SS, Jacks T, Weinberg RA. Cell. 2008 Jul 11. 134(1):62-73. 10.1016/j.cell.2008.06.006 PubMed 18614011
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