Skip to main content
Addgene

pCA528 IST1, residues 316-366, C-Cys
(Plasmid #191234)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191234 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pCA528
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IST1
  • Species
    H. sapiens (human)
  • Mutation
    Residues 316-366, contains nonnative C-terminus for labeling
  • Entrez Gene
    IST1 (a.k.a. CHMP8, OLC1)
  • Promoter T7
  • Tag / Fusion Protein
    • HIS-SUMO (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCA528 IST1, residues 316-366, C-Cys was a gift from Wesley Sundquist (Addgene plasmid # 191234 ; http://n2t.net/addgene:191234 ; RRID:Addgene_191234)

  • For your References section:

    Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission. Wenzel DM, Mackay DR, Skalicky JJ, Paine EL, Miller MS, Ullman KS, Sundquist WI. Elife. 2022 Sep 15;11:e77779. doi: 10.7554/eLife.77779. 10.7554/eLife.77779 PubMed 36107470
Related items: