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Addgene

pCAG-AsCas12a-Plus-mCherry
(Plasmid #191231)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191231 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PbluSKP
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AsCas12a-Plus-P2A-mCherry
  • Species
    Other
  • Mutation
    E174R, R951K, R955A

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-AsCas12a-Plus-mCherry was a gift from Zhili Rong (Addgene plasmid # 191231 ; http://n2t.net/addgene:191231 ; RRID:Addgene_191231)

  • For your References section:

    Engineered Cas12a-Plus nuclease enables gene editing with enhanced activity and specificity. Huang H, Huang G, Tan Z, Hu Y, Shan L, Zhou J, Zhang X, Ma S, Lv W, Huang T, Liu Y, Wang D, Zhao X, Lin Y, Rong Z. BMC Biol. 2022 Apr 25;20(1):91. doi: 10.1186/s12915-022-01296-1. 10.1186/s12915-022-01296-1 PubMed 35468792
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