shCD44-2 pRRL
(Plasmid #19123)

• Depositing Lab: Bob Weinberg
• Publication: Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Backbone

• Vector backbone: pRRL-U6-siluc-cppt-hPGKGFPSIN
• Backbone size w/o insert (bp): 7247
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C, grows slowly.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: small hairpin RNA against CD44
• Alt name: CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
• Species: H. sapiens (human)
• Insert Size (bp): 60
• Entrez Gene: CD44 (a.k.a. CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: U6-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

shRNA to human CD44 (should target all isoforms). Subcloned via NdeI-BamHI frm pLKO1.Puro. This insert replaced identical sequence present in pRRL-EGFP and added the shRNA sequence

shRNA oligo sequence: sense:5'-CCGGTATGCAATGTGCTACTGATTGTCTCGAGACAATCAGTAGCACATTGCATTTTTTG-3'

Please note that the shRNA oligo present in this plasmid is referred to as shCD44-3 in the associated publication.