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Chinchilla+N122D–insM
(Plasmid #191227)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191227 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac Dual
  • Backbone size w/o insert (bp) 5238
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    ATP1A1
  • Species
    Chinchilla lanigera
  • Insert Size (bp)
    3072
  • Mutation
    removed methionine at 122 and replaced asparagine at 129 with aspartic acid in the protein sequence
  • GenBank ID
    XM_005389040
  • Promoter PH

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ATTCCGGATTATTCATACCGTCCCACCATCG
  • 3′ sequencing primer GTGGTATGGCTGATTATGATCCTCTAGTACTTCTC
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ATP1B1
  • Species
    Chinchilla lanigera
  • Insert Size (bp)
    921
  • GenBank ID
    XM_005398203
  • Promoter p10

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site PaeI (unknown if destroyed)
  • 5′ sequencing primer CGGGTTCCTTCCGGTATTGTCTCCTTC
  • 3′ sequencing primer ACGGACCTTTAATTCAACCCAACACAATATA
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Chinchilla+N122D–insM was a gift from Susanne Dobler (Addgene plasmid # 191227 ; http://n2t.net/addgene:191227 ; RRID:Addgene_191227)

  • For your References section:

    Epistatic effects between amino acid insertions and substitutions mediate toxin-resistance of vertebrate Na+, K+-ATPases. Mohammadi S, Ozdemir HI, Ozbek P, Sumbul F, Stiller J, Deng Y, Crawford AJ, Rowland HM, Storz JF, Andolfatto P, Dobler S. Mol Biol Evol. 2022 Dec 6:msac258. doi: 10.1093/molbev/msac258. 10.1093/molbev/msac258 PubMed 36472530
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