CD44P pGL3
(Plasmid #19122)

• Depositing Lab: Bob Weinberg
• Publication: Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Backbone

• Vector backbone: pGL3-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Non viral cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: CD44 promoter
• Alt name: CDW44, CSPG8, ECMR-III, HCELL, IN, LHR, MC56, MDU2, MDU3, MGC10468, MIC4, MUTCH-I, Pgp1
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Entrez Gene: CD44 (a.k.a. CDW44, CSPG8, ECM-III, ECMR-III, H-CAM, HCELL, HUTCH-1, HUTCH-I, Hermes-1, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PCR amplified from genomic DNA (human HMLE cells). Contains CD44 promoter, 2021bp upstream of translation initiation site.