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Addgene

pCALNL-tdTomato-2A-mSyp::EGFP
(Plasmid #191213)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191213 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCALNL
  • Backbone manufacturer
    Connie Cepko (Addgene plasmid # 13769)
  • Backbone size w/o insert (bp) 6107
  • Total vector size (bp) 9288
  • Vector type
    Mammalian Expression ; pCAGEN

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tdTomato-T2A-mSyp::EGFP
  • Alt name
    tdTomato
  • Alt name
    mouse Synaptophysin
  • Alt name
    enhanced green fluorescent protein
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3181
  • GenBank ID
    NM_009305.2
  • Entrez Gene
    Syp (a.k.a. A230093K24Rik, Syn, p38)
  • Tag / Fusion Protein
    • mSyp::EGFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer aaggcctgtccgatgtgaag
  • 3′ sequencing primer ttcaggaagccaaacaccac
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Suggested primers to sequence the insert:
5' ccttcttgacgagttcttctg 3'
5' acgtctccgcatgtcagaag 3'
5' aaggcctgtccgatgtgaag 3'
5' ttcaggaagccaaacaccac 3'
5' GCTTGCCGTAGGTGGCATC 3'
5' CAGGAAACAGCTATGAC 3'

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCALNL-tdTomato-2A-mSyp::EGFP was a gift from Theofanis Karayannis (Addgene plasmid # 191213 ; http://n2t.net/addgene:191213 ; RRID:Addgene_191213)

  • For your References section:

    Sparse postnatal labeling and quantification of superficial cortical cell synapses in the mouse neocortex. Gesuita L, Argunsah AO, Karayannis T. STAR Protoc. 2022 Nov 11;3(4):101837. doi: 10.1016/j.xpro.2022.101837. eCollection 2022 Dec 16. 10.1016/j.xpro.2022.101837 PubMed 36386881
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