pLEX-307_LgBiT
(Plasmid
#191211)
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PurposeLentiviral-mediated stable expression of LgBiT for slit nanoluciferase complementation assays in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191211 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLEX-307
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Backbone manufacturerDavid Root
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Vector typeLentiviral, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLgBiT (large bit)
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SpeciesOplophorus gracilirostris
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MutationAB030246.1 (mutated in PMID 26569370)
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GenBank IDAB030246.1 (mutated in PMID 26569370)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLEX-307_LgBiT was a gift from Mohan Babu (Addgene plasmid # 191211 ; http://n2t.net/addgene:191211 ; RRID:Addgene_191211)