pAAV-7xTRE-3xeGFP
(Plasmid
#191206)
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PurposeGFP expression under the control of TRE promotor
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191206 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4463
- Total vector size (bp) 6746
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsGrow for minimum time necessary. Do not store colonies on plate.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3X GFP
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Insert Size (bp)2283
- Promoter TRE
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byJanelia MouseLight Project, Jayaram Chandrashekar, and Kimberly Ritola
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-7xTRE-3xeGFP was a gift from Janelia Viral Tools (Addgene plasmid # 191206 ; http://n2t.net/addgene:191206 ; RRID:Addgene_191206) -
For your References section:
A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues. Glaser AK, Bishop KW, Barner LA, Susaki EA, Kubota SI, Gao G, Serafin RB, Balaram P, Turschak E, Nicovich PR, Lai H, Lucas LAG, Yi Y, Nichols EK, Huang H, Reder NP, Wilson JJ, Sivakumar R, Shamskhou E, Stoltzfus CR, Wei X, Hempton AK, Pende M, Murawala P, Dodt HU, Imaizumi T, Shendure J, Beliveau BJ, Gerner MY, Xin L, Zhao H, True LD, Reid RC, Chandrashekar J, Ueda HR, Svoboda K, Liu JTC. Nat Methods. 2022 May;19(5):613-619. doi: 10.1038/s41592-022-01468-5. Epub 2022 May 11. 10.1038/s41592-022-01468-5 PubMed 35545715