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pAAV-TRE-FRT-2xtTA
(Plasmid #191205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191205 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4805
  • Total vector size (bp) 6278
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow for minimum time necessary. Do not store colonies on plate.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    2xtTA
  • Insert Size (bp)
    1473
  • Promoter TRE

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Janelia MouseLight Project, Jayaram Chandrashekar, and Kimberly Ritola

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-TRE-FRT-2xtTA was a gift from Janelia Viral Tools (Addgene plasmid # 191205 ; http://n2t.net/addgene:191205 ; RRID:Addgene_191205)

  • For your References section:

    A hybrid open-top light-sheet microscope for versatile multi-scale imaging of cleared tissues. Glaser AK, Bishop KW, Barner LA, Susaki EA, Kubota SI, Gao G, Serafin RB, Balaram P, Turschak E, Nicovich PR, Lai H, Lucas LAG, Yi Y, Nichols EK, Huang H, Reder NP, Wilson JJ, Sivakumar R, Shamskhou E, Stoltzfus CR, Wei X, Hempton AK, Pende M, Murawala P, Dodt HU, Imaizumi T, Shendure J, Beliveau BJ, Gerner MY, Xin L, Zhao H, True LD, Reid RC, Chandrashekar J, Ueda HR, Svoboda K, Liu JTC. Nat Methods. 2022 May;19(5):613-619. doi: 10.1038/s41592-022-01468-5. Epub 2022 May 11. 10.1038/s41592-022-01468-5 PubMed 35545715
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