pAAV-CAG-FRT-Rev-3xGFP
(Plasmid
#191204)
-
PurposeFlpO dependent GFP expression
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191204 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepAAV
- Backbone size w/o insert (bp) 5032
- Total vector size (bp) 7306
-
Vector typeMammalian Expression, AAV
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Growth instructionsGrow for minimum time necessary. Do not store colonies on plate.
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name3X GFP
-
Insert Size (bp)2274
- Promoter CAG
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer N/A
- 3′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
A portion of this plasmid was derived from a plasmid made byJanelia MouseLight Project, Jayaram Chandrashekar, and Kimberly Ritola
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAV-CAG-FRT-Rev-3xGFP was a gift from Janelia Viral Tools (Addgene plasmid # 191204 ; http://n2t.net/addgene:191204 ; RRID:Addgene_191204) -
For your References section:
Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain. Winnubst J, Bas E, Ferreira TA, Wu Z, Economo MN, Edson P, Arthur BJ, Bruns C, Rokicki K, Schauder D, Olbris DJ, Murphy SD, Ackerman DG, Arshadi C, Baldwin P, Blake R, Elsayed A, Hasan M, Ramirez D, Dos Santos B, Weldon M, Zafar A, Dudman JT, Gerfen CR, Hantman AW, Korff W, Sternson SM, Spruston N, Svoboda K, Chandrashekar J. Cell. 2019 Sep 19;179(1):268-281.e13. doi: 10.1016/j.cell.2019.07.042. Epub 2019 Sep 5. 10.1016/j.cell.2019.07.042 PubMed 31495573
