pAAV-CAG-FRT-Rev-3xGFP
(Plasmid #191204)

• Purpose: FlpO dependent GFP expression
• Depositing Lab: Janelia Viral Tools
• Publication: Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. doi: 10.1016/j.cell.2019.07.042. Epub 2019 Sep 5.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 5032
• Total vector size (bp): 7306
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: Grow for minimum time necessary. Do not store colonies on plate.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3X GFP
• Insert Size (bp): 2274
• Promoter: CAG

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • CAG-FRT-Rev-3xGFP.gbk
• A portion of this plasmid was derived from a plasmid made by: Janelia MouseLight Project, Jayaram Chandrashekar, and Kimberly Ritola

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry