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Addgene

pAAV-Syn-Flex-FlpO
(Plasmid #191202)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191202 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pAAV
  • Backbone size w/o insert (bp) 4901
  • Total vector size (bp) 6173
  • Vector type
    Mammalian Expression, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    Grow for minimum time necessary. Do not store colonies on plate.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FlpO
  • Insert Size (bp)
    1272
  • Promoter hSyn

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Janelia MouseLight Project, Jayaram Chandrashekar, and Kimberly Ritola

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Syn-Flex-FlpO was a gift from Janelia Viral Tools (Addgene plasmid # 191202 ; http://n2t.net/addgene:191202 ; RRID:Addgene_191202)
  • For your References section:

    Reconstruction of 1,000 Projection Neurons Reveals New Cell Types and Organization of Long-Range Connectivity in the Mouse Brain. Winnubst J, Bas E, Ferreira TA, Wu Z, Economo MN, Edson P, Arthur BJ, Bruns C, Rokicki K, Schauder D, Olbris DJ, Murphy SD, Ackerman DG, Arshadi C, Baldwin P, Blake R, Elsayed A, Hasan M, Ramirez D, Dos Santos B, Weldon M, Zafar A, Dudman JT, Gerfen CR, Hantman AW, Korff W, Sternson SM, Spruston N, Svoboda K, Chandrashekar J. Cell. 2019 Sep 19;179(1):268-281.e13. doi: 10.1016/j.cell.2019.07.042. Epub 2019 Sep 5. 10.1016/j.cell.2019.07.042 PubMed 31495573