ALIX(1-702)-mNeonGreen
(Plasmid #191193)

• Purpose: Truncated form of ALIX that lacks C-terminal proline-rich domain; C-terminally tagged with mNeonGreen.
• Depositing Lab: Jin Zhang
• Publication: Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi: 10.1126/sciadv.adg3913. Epub 2023 Jul 14.

Backbone

• Vector backbone: pcDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5396
• Total vector size (bp): 8225
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ALIX (1-702)
• Alt name: PDCD6IP (1-702)
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 2829
• Mutation: Deletion of ALIX C-terminal PRD.
• Entrez Gene: PDCD6IP (a.k.a. AIP1, ALIX, DRIP4, HP95, MCPH29)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHIS (N terminal on insert)
  • mNeonGreen (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: BGH reverse

Resource Information

• Supplemental Documents:
  • pcDNA3.1(+) ALIX(1-702)-mNG.gbk
• A portion of this plasmid was derived from a plasmid made by: ALIX (1-702) cDNA was provided by Lalit Deshmukh (UCSD)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry