shp53 pLKO.1 puro
(Plasmid #19119)

• Purpose: 3rd gen lentiviral vector for knocking down p53 gene expression
• Depositing Lab: Bob Weinberg
• Publication: Godar et al Cell. 2008 Jul 11. 134(1):62-73.

Backbone

• Vector backbone: pLKO.1 puro
• Backbone manufacturer: GenHunter
• Backbone size w/o insert (bp): 7032
• Vector type: Mammalian Expression, Lentiviral, RNAi
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use Stbl3 or HB101 to reduce chance of recombination.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: small hairpin RNA against tumor protein p53
• Alt name: p53, FLJ92943, LFS1, TRP53
• Species: H. sapiens (human)
• Entrez Gene: TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: LKO.1 5'

Resource Information

• Addgene Notes:
  • shRNA oligo sequence
• Reference:
  • Addgene's pLKO.1 protocol
• Articles Citing this Plasmid:
  • 44 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloned by Watmick RS.