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Addgene

EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
(Plasmid #191175)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191175 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    FLP-IN
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CMV-TO:mCherry:s70587:EGFP (frameshifted)
  • Species
    Synthetic
  • Mutation
    EGFP is frameshifted
  • Promoter CMV-TO

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please see https://rnasensing.bio/ for more details. Please visit https://www.biorxiv.org/content/10.1101/2022.01.28.478207v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN) was a gift from Xiaojing Gao (Addgene plasmid # 191175 ; http://n2t.net/addgene:191175 ; RRID:Addgene_191175)
  • For your References section:

    Modular, programmable RNA sensing using ADAR editing in living cells. Kaseniit KE, Katz N, Kolber NS, Call CC, Wengier DL, Cody WB, Sattely ES, Gao XJ. Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 10.1038/s41587-022-01493-x. 10.1038/s41587-022-01493-x PubMed 36198772