EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN)
(Plasmid
#191175)
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PurposeMammalian expression of EK0209 with frameshift mutation (EGFP is frameshifted).
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 191175 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFLP-IN
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCMV-TO:mCherry:s70587:EGFP (frameshifted)
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SpeciesSynthetic
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MutationEGFP is frameshifted
- Promoter CMV-TO
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see https://rnasensing.bio/ for more details. Please visit https://www.biorxiv.org/content/10.1101/2022.01.28.478207v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EK0261 CMV-TO-mCherry-SG(s70587)SG-fs(EGFP) (FLP-IN) was a gift from Xiaojing Gao (Addgene plasmid # 191175 ; http://n2t.net/addgene:191175 ; RRID:Addgene_191175) -
For your References section:
Modular, programmable RNA sensing using ADAR editing in living cells. Kaseniit KE, Katz N, Kolber NS, Call CC, Wengier DL, Cody WB, Sattely ES, Gao XJ. Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 10.1038/s41587-022-01493-x. 10.1038/s41587-022-01493-x PubMed 36198772