EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN)
(Plasmid #191164)

• Purpose: Similar to EK0285 but has additional restriction sites around the sensor sequence. Recommended for cloning new sensors when the presence of MS2 sequences are not relevant (use EK0438 if MS2 sequences
• Depositing Lab: Xiaojing Gao
• Publication: Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 10.1038/s41587-022-01493-x.

Backbone

• Vector backbone: FLP-IN
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mCherry:s70587:EGFP (additional cutsites)
• Species: Synthetic
• Mutation: WT
• Promoter: SFFV

Cloning Information

• Cloning method: Unknown

Resource Information

• Supplemental Documents:
  • EK0409 SFFV-mCherry-SGc(s70587)cSG-EGFP (FLP-IN).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

Please see https://rnasensing.bio/ for more details. Please visit https://www.biorxiv.org/content/10.1101/2022.01.28.478207v1 for bioRxiv preprint.