EK0379 CMV-TO-EGFP-stop-t70587 (PB)
(Plasmid
#191154)
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PurposeExpression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191154 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonePiggyBac
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP-t70587
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SpeciesSynthetic
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MutationWT
- Promoter CMV-TO
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see https://rnasensing.bio/ for more details. Please visit https://www.biorxiv.org/content/10.1101/2022.01.28.478207v1 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EK0379 CMV-TO-EGFP-stop-t70587 (PB) was a gift from Xiaojing Gao (Addgene plasmid # 191154 ; http://n2t.net/addgene:191154 ; RRID:Addgene_191154) -
For your References section:
Modular, programmable RNA sensing using ADAR editing in living cells. Kaseniit KE, Katz N, Kolber NS, Call CC, Wengier DL, Cody WB, Sattely ES, Gao XJ. Nat Biotechnol. 2022 Oct 5. pii: 10.1038/s41587-022-01493-x. doi: 10.1038/s41587-022-01493-x. 10.1038/s41587-022-01493-x PubMed 36198772
