Skip to main content
Addgene

pVioCD53
(Plasmid #191130)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191130 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSB1A3-J23105
  • Backbone size w/o insert (bp) 2301
  • Total vector size (bp) 4886
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    use BL21A1 for protein expression
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    vioC
  • Species
    Synthetic
  • Insert Size (bp)
    1284
  • Entrez Gene
    vioC (a.k.a. CV_3272)
  • Promoter J23105 constitutive promoter

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    vioD truncated
  • Alt name
    vioD
  • Species
    Synthetic
  • Insert Size (bp)
    790
  • Mutation
    C-terminal truncation from 264th amino acid

Gene/Insert 3

  • Gene/Insert name
    dMCP off-frame
  • Alt name
    MCP
  • Alt name
    dMCP
  • Species
    Synthetic
  • Insert Size (bp)
    291

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVioCD53 was a gift from Ariel Lindner (Addgene plasmid # 191130 ; http://n2t.net/addgene:191130 ; RRID:Addgene_191130)

  • For your References section:

    Spatial engineering of E. coli with addressable phase-separated RNAs. Guo H, Ryan JC, Song X, Mallet A, Zhang M, Pabst V, Decrulle AL, Ejsmont P, Wintermute EH, Lindner AB. Cell. 2022 Sep 29;185(20):3823-3837.e23. doi: 10.1016/j.cell.2022.09.016. 10.1016/j.cell.2022.09.016 PubMed 36179672
Related items: