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pPN-mKate (PT7LacO-PN-mKate)
(Plasmid #191126)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191126 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7-LacO
  • Backbone size w/o insert (bp) 4145
  • Total vector size (bp) 4466
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein N
  • Alt name
    λN
  • Alt name
    PN
  • Species
    Synthetic
  • Insert Size (bp)
    321
  • Promoter T7-lac inducible promoter
  • Tag / Fusion Protein
    • mKate

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPN-mKate (PT7LacO-PN-mKate) was a gift from Ariel Lindner (Addgene plasmid # 191126 ; http://n2t.net/addgene:191126 ; RRID:Addgene_191126)

  • For your References section:

    Spatial engineering of E. coli with addressable phase-separated RNAs. Guo H, Ryan JC, Song X, Mallet A, Zhang M, Pabst V, Decrulle AL, Ejsmont P, Wintermute EH, Lindner AB. Cell. 2022 Sep 29;185(20):3823-3837.e23. doi: 10.1016/j.cell.2022.09.016. 10.1016/j.cell.2022.09.016 PubMed 36179672
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