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Addgene

pCS2-EcYRS-EGFP*
(Plasmid #191118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
  • Alt name
    EcYRS - T2A - EGFP
  • Species
    Synthetic; E. coli
  • Insert Size (bp)
    2127
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-EcYRS-EGFP* was a gift from Han Xiao (Addgene plasmid # 191118 ; http://n2t.net/addgene:191118 ; RRID:Addgene_191118)

  • For your References section:

    Expanding the eukaryotic genetic code with a biosynthesized 21st amino acid. Wu KL, Moore JA, Miller MD, Chen Y, Lee C, Xu W, Peng Z, Duan Q, Phillips GN Jr, Uribe RA, Xiao H. Protein Sci. 2022 Oct;31(10):e4443. doi: 10.1002/pro.4443. 10.1002/pro.4443 PubMed 36173166
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