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Addgene

pLei-sfGFP-V2*D134*
(Plasmid #191111)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191111 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLei
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    superfolder green fluorescence protein
  • Alt name
    sfGFP
  • Species
    Synthetic
  • Insert Size (bp)
    744
  • Mutation
    changing V2 & D134 to TAG
  • Tag / Fusion Protein
    • His tag (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLei-sfGFP-V2*D134* was a gift from Han Xiao (Addgene plasmid # 191111 ; http://n2t.net/addgene:191111 ; RRID:Addgene_191111)

  • For your References section:

    Expanding the eukaryotic genetic code with a biosynthesized 21st amino acid. Wu KL, Moore JA, Miller MD, Chen Y, Lee C, Xu W, Peng Z, Duan Q, Phillips GN Jr, Uribe RA, Xiao H. Protein Sci. 2022 Oct;31(10):e4443. doi: 10.1002/pro.4443. 10.1002/pro.4443 PubMed 36173166
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