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Addgene

Dom34 K45A
(Plasmid #19111)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19111 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a(+)
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Protein DOM34
  • Alt name
    Saccharomyces cerevisiae
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    936
  • Mutation
    K45A
  • Entrez Gene
    DOM34 (a.k.a. YNL001W)
  • Tag / Fusion Protein
    • C tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer t7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dom34 K45A was a gift from SeWon Suh (Addgene plasmid # 19111 ; http://n2t.net/addgene:19111 ; RRID:Addgene_19111)

  • For your References section:

    Structural and functional insights into dom34, a key component of no-go mRNA decay. Lee HH, Kim YS, Kim KH, Heo I, Kim SK, Kim O, Kim HK, Yoon JY, Kim HS, Kim do J, Lee SJ, Yoon HJ, Kim SJ, Lee BG, Song HK, Kim VN, Park CM, Suh SW. Mol Cell. 2007 Sep 21. 27(6):938-50. 10.1016/j.molcel.2007.07.019 PubMed 17889667
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