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pAAV2-UbC-3xNLS-mScarlet-I
(Plasmid #191099)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 191099 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-SIBR-anti_I
  • Backbone manufacturer
    Chung K-H et al., Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR 2006 vol 24
  • Backbone size w/o insert (bp) 5322
  • Total vector size (bp) 6130
  • Modifications to backbone
    The anti-luciferase cassette was deleted
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xNLS-mScarlet-I
  • Species
    Synthetic
  • Insert Size (bp)
    798
  • Mutation
    optimized to human codon usage
  • Promoter UbC
  • Tag / Fusion Protein
    • Three repeats of the nuclear localizzation sequences from SV40 fused to mScarlet-I.FPbase ID: 6VVTK (N terminal on insert) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, NheI (not destroyed)
  • 3′ cloning site None (destroyed during cloning)
  • 5′ sequencing primer pCAF-F
  • 3′ sequencing primer hGH-PA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV2-UbC-3xNLS-mScarlet-I was a gift from Murray Blackmore & Pantelis Tsoulfas (Addgene plasmid # 191099 ; http://n2t.net/addgene:191099 ; RRID:Addgene_191099)

  • For your References section:

    Brain-wide analysis of the supraspinal connectome reveals anatomical correlates to functional recovery after spinal injury. Wang Z, Romanski A, Mehra V, Wang Y, Brannigan M, Campbell BC, Petsko GA, Tsoulfas P, Blackmore MG. Elife. 2022 Jul 15;11. pii: 76254. doi: 10.7554/eLife.76254. 10.7554/eLife.76254 PubMed 35838234
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